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Image Search Results
Journal: Journal of Cellular and Molecular Medicine
Article Title: Modulation of Wnt/β‐Catenin Pathway by Aesculus hippocastanum Extract Enhances Temozolomide Sensitivity in Glioblastoma Cells
doi: 10.1111/jcmm.70979
Figure Lengend Snippet: HCE and TMZ combination inhibits Wnt/β‐catenin signalling and modulates glioma stemness and differentiation markers in U87 cells. U87 cells were treated with HCE (500 μg/mL), TMZ (100 μM), or their combination, for 48 h. Western blot analysis of β‐catenin and Wnt‐1 was performed (A, B; A1, B1). ELISA kit for phospho‐GSK‐3β (Ser9) and total GSK‐3β was shown in (C). Nestin (D–H) and β3‐tubulin (I–M) were assessed by IF. Images of IF were captured at 40× magnification. Data are representative of three independent experiments. One‐Way ANOVA. (A1) * p < 0.05 versus Control; *** p < 0.001 versus Control; # p < 0.05 versus TMZ. (B1) * p < 0.05 versus Control; *** p < 0.001 versus Control; ## p < 0.01 versus TMZ. (C) * p < 0.05 versus Control; *** p < 0.001 versus Control; # p < 0.05 versus TMZ. (H) ** p < 0.01 versus Control; *** p < 0.001 versus Control; ## p < 0.01 versus TMZ. (M) ** p < 0.01 versus Control; *** p < 0.001 versus Control; ## p < 0.01 versus TMZ.
Article Snippet: The levels of phospho‐GSK‐3β (Ser9) and total GSK‐3β (
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Control